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Fractions from the above fractionation were dissolved with solvent A (0.1% formic acid in H 2 O) and separated with a homemade reversed-phase 25-cm analytical column (75 μm ID, 1.9 μm C18) in a 65-min gradient from 5% to 50% solvent B (0.1% formic acid in 80% ACN) using the EASY-nLC 1200 system (Thermo Fisher Scientific)
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doi: 10.1111/j.1365-3040.2011.02400.x
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